Faculté de médecine Faculté de médecine

Widefield Microscopy (4 systems)

Video time-lapse + Temp Controller + CO2 (Tel. 94 778)

Capabilities

  • Fluorescence/ transmission widefield microscope : DAPI, CFP, YFP, GFP, mCherry, Cy5
  • 2 cameras (one monochrome and one color)
  • DIC for objectives 40x and 63x
  • Temperature, CO2 control
  • Time-lapse video-microscopy (live cells).
  • Imaging fixed samples (culture cells)
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Detailed specifications

Microscope

  • Zeiss Axio Observer Z1 with Definite Focus 2

Objectives

  • Achroplan  4x / 0.10 WD 11.1 mm
  • EC Plan-Neofluar 10x / 0.30, Ph1  WD 5.2 mm  (Definite Focus 2 compatible)
  • EC Plan-Neofluar 20x / 0.50, Ph2  WD 2.0 mm  (Definite Focus 2 compatible)
  • EC Plan-Apochromat 20x/0.8 WD 0.55 mm (Definite Focus 2 compatible)
  • Plan Apochromat 40x / 1.4 Oil DIC III (UV) VIS-IR  (Definite Focus 2 compatible)
  • Plan Apochromat 63x / 1.4 Oil DIC III (Definite Focus 2 compatible)

 

Objective

Axiocam 506 mono

micron/pixel
4x 1.1
4x optovar 1.6 x 0.713
10x 0.44
10x optovar 1.6x 0.285
20x 0.220
20x optovar 1.6x 0.143
40x 0.110
40x optovar 1.6x 0.071
63x 0.069
63x optovar 1.6x 0.045

 

Excitation with Colibri 7 type R[G/Y]CBV-UV :

  1. 385 / 30 nm DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue
  2. 423 / 44 nm Pacific Blue, Lucifer Yellow, Alexa Fluor 433, eCFP, Cerulean
  3. 469 / 38 nm FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, Honeydew, FITC / Fluorescein, Kaede (green / red), PerCP, YoYo-1,FuraRed
  4. 511 / 44 nm Rhodamine 123, Fluo-4, Oregon Green BAPTA, Sytox Green, eYFP, FM4-64, Eosin / HE, Acridine Orange, JC1, Bodipy FL, Propidium Iodide, Spectrum Green, Calcium Green
  5. 555 / 30 nm TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange
  6. 591 / 27 nm MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR
  7. 631 / 33 nm Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N

Filters turret microscope :

  1. Empty
  2. FT 495   FITC, GFP
  3. Quad Band : Semrock DAPI / GFP / RFP / Cy5   Quad_band_filter.pdf
  4. T 610  for Alexa 594
  5. FT 570   Cy3, RFP
  6. DBP 450+538  CFP-YFP

Emission filter :

  1. DIC Analyzer
  2. DAPI  435 / 40
  3. GFP  525 / 50
  4. RFP  BP 575-640
  5. Cy5  700 / 75
  6. Alexa 594  647/57
  7. CFP 470/24
  8. YFP 585/65

2 Detector systems

  • Axiocam 506 mono  2752 x 2208 pixels  14 bit
  • Axiocam 305 colour 5Megapixel 2464 x 2056 12 bits

Computer

  • HP Z6 G4 Workstation
  • Zen 2.6