My project is to decipher the mechanism of ESCRTs dependent intra-luminal vesicles (ILV) formation in endosomes, a process which requires membrane deformation and fission by ESCRT machinery. For that, by combining in vitro and in cellulo experiments, I want both to determine the ESCRT subunits which are specifically involved in this process but also analyse the effect of physical membrane properties modulation, such as membrane tension on ESCRT recruitment and activity. We saw already a link between ESCRT and membrane tension which provides a good way to activate ESCRT and better understand, using different techniques such as live imaging or 3D correlative light electron microscopy the functioning of this machinery.