Antibody Discovery
Our main objective is to allow the academic community to gain access to recombinant antibodies at an affordable price. We aim to select within 12 weeks at least one recombinant antibody against your favorite protein at a cost of CHF 4'000 (internal clients from the University of Geneva and of other Swiss universities have preferential prices).
How to order a new recombinant antibody :
Indicate the name (with UniProt ID) and species of your target protein, as well as any special requirements about the desired epitope.
2. Our standard procedure involves the use of peptides (30-40 aa) or a purified protein (provided by you, 50-200 µg) to select recombinant antibodies against a given protein. We can also produce a tagged protein antigen upon request (1'000 CHF).
3. By the end of the selection, every selected antibody is checked by ELISA against the antigen. The whole selection procedure should take around 12 weeks.
Be aware that the antibodies produced will recognize what they were selected for: if your aim is toselect an antibody that recognizes the native protein by immunofluorescence, then the selection should ideally be against the full-length protein, or a properly folded domain. If your objective is rather to use the antibody to recognize a denatured protein by western blot, then peptides can also be used.
We provide the antibodies as non-purified mini-antibodies with the scFv antigen-binding site fused to an Fc . Our current offer of Fc portions are: rabbit IgG; human IgG1 and IgG2; mouse IgG1, IgG2A; guinea pig IgG.
4. You will be charged 4'000 CHF when you pass your order. While our experience is that we select at least one specific antibody against most targets, we cannot guarantee a 100% rate of success.
5. The antibodies developed for you will be made available to the scientific community!
Please let us know if you have any reservation about this.
If you want to cite us on your publication, you can use the following: "A recombinant anti-XXXX antibody (ABCD_RBXXX) was generated by the Geneva Antibody Facility (https://www.unige.ch/medecine/antibodies/)"
Important: be aware that the antibodies developed by the facility are restricted to fundamental research use. Any commercial development is subject to restrictions and must be negotiated with the University of Geneva.
